DNA Sequence Resembling vanA and vanB in the Vancomycin-Resistant Biopesticide Bacillus popilliae

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The biopesticide Paenibacillus popilliae has a vancomycin resistance gene cluster homologous to the enterococcal VanA vancomycin resistance gene cluster.

We have previously identified, in Paenibacillus popilliae, a 708-bp sequence which has homology to the sequence of the enterococcal vanA gene. We have performed further studies revealing five genes encoding homologues of VanY, VanZ, VanH, VanA, and VanX in P. popilliae. The predicted amino acid sequences are similar to those in VanA vancomycin-resistant enterococci: 61% identity for VanY, 21% f...

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VanA and VanB Positive Vancomycin-resistant Staphylococcus aureus Among Clinical Isolates in Shiraz, South of Iran.

OBJECTIVE The purpose of this study was to determine the prevalence of vancomycin-resistant Staphylococcus aureus isolated from clinical samples in Shiraz hospitals. METHODS From March to December 2012, 100 S. aureus isolates (mainly from wound and blood) were collected from three hospitals in Shiraz, south of Iran. After identification of Staphylococcus aureus by biochemical, microbiological...

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Surveillance of Vancomycin-resistant Enterococci colonization with GeneXpert vanA/vanB test and culture method

Objectives: Early detection of vancomycin resistant enterococci (VRE) colonization in hospitalized patients plays an important role in controlling enterococcal infections. For this purpose, the detection of VRE from rectal swab specimens can be performed with various methods. Methods: In this study, VRE colonization in the rectal swab samples of patients from intensive care units, burn unit, an...

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Evaluation of the EVIGENE VRE detection kit for detection of vanA and vanB genes in vancomycin-resistant enterococci.

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Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs.

We compared the performance characteristics of a real-time PCR method, the LightCycler vanA/vanB detection assay (Roche Diagnostics Corporation, Indianapolis, Ind.) to that of Enterococcosel agar (BBL, Sparks, Md.) for direct detection of vancomycin-resistant enterococci (VRE) from 894 perianal stool swabs. For 421 of 894 swabs, the result for LightCycler PCR was compared to an Enterococcosel p...

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ژورنال

عنوان ژورنال: Journal of Infectious Diseases

سال: 1998

ISSN: 0022-1899,1537-6613

DOI: 10.1086/517480